12/7/2023 0 Comments Download blue glow worm![]() ![]() Wood KV, Lam YA, Seliger HH, McElroy WD.Stepwise removal of the C-terminal 12 amino acids of firefly luciferase results in graded loss of activity. Removal of twelve C-terminal amino acids from firefly luciferase abolishes activity. Sala-Newby G, Kalsheker N, Campbell AK. ![]() Real-time analysis of the transcriptional regulation of HIV and hCMV promoters in single mammalian cells. White MR, Masuko M, Amet L, Elliott G, Braddock M, Kingsman AJ, Kingsman SM.Engineering firefly luciferase as an indicator of cyclic AMP-dependent protein kinase in living cells. This may not be the complete list of references from this article. The Full Text of this article is available as a PDF (894K). It was concluded that the molecular and biochemical properties were not sufficient to explain the glowing or flashing of the two beetles Lampyris and Photinus. The pH optimum and the Km values for ATP and luciferin were similar for both Photinus and Lampyris luciferases, although the light emitted by the latter shifted towards the blue and was less stable at 37 degrees C. Engineering Lampyris luciferase in vitro showed that the C-terminal peptide containing 12 amino acids in Photinus and 9 amino acids in Lampyris was essential for bioluminescence. In contrast, Lampyris luciferase had less sequence similarity to the luciferases of the click beetle Pyrophorus, at 48%. The protein was closely related to those of other lampyrid beetles, the similarity to Photinus pyralis luciferase being 84% and to Luciola 67%. The luciferase was a 547-residue protein, as deduced from the nucleotide sequence. A full-length clone encoding Lampyris noctiluca (British glow-worm) luciferase was isolated from a complementary DNA (cDNA) expression library constructed with MRNA extracted from light organs. ![]()
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